KMID : 0613820150250070733
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Journal of Life Science 2015 Volume.25 No. 7 p.733 ~ p.739
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Development of Axenic Culture and Astaxanthin Production in Microalgae
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Son Min-Chang
Lee Dong-Jun Park Se-Jin Kim Min-Sung Lee Chul-Won An Won-Gun
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Abstract
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Microalgae are a renewable natural resource that requires only sunlight, carbon dioxide, phosphorus, and nitrogen for rapid growth. They produce a broad variety of basic chemical substances¡ªsuch as vitamins, fatty acids and carotenoids£that have high added value potential for the pharmaceutical and food industries. The aim of this study was to develop axenic culture and to establish a cell growth assay for microalgae. A further experiment was carried out to determine the yield of astaxanthin derived from microalgae. The axenic culture was developed using a mixture of antibiotics [ampicillin (100 ¥ìg/ml), streptomycin (10 ¥ìg/ml), chloramphenicol (10 ¥ìg/ml), penicillin (10 ¥ìg/ml), neomycin (50 ¥ìg/ml), gentamycin (50 ¥ìg/ml), kanamycin (10 ¥ìg/ml), and nystatin (1.5 ¥ìg/ml)] and then used to extract a variety of useful components from the microalgae. The optimal concentration for the antibiotic mixture was 1-3 percent. A spectrophotometric cell growth assay was also established. Astaxanthin was extracted from Haematococus lacustris with a yield of 1.9¡¿10-3 ¥ìg/l per 1 ml of culture medium. In conclusion, the axenic culture method developed here allows extraction of high-quality astaxanthin and other useful components from microalgae.
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KEYWORD
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Antibiotics, astaxanthin, axenic culture, cell growth assay, microalgae
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